Best Solution for Digital Histology Product Catalogue * Premade Recipient Block *Manual TissueMicroarrayer * AutomatedTissueMicroarrayers * Recipient BlockMoldKit * ParaffinBlockTrimmer www.unitma.com UnitmaCo., Ltd. 3F, Dongil B/D, 36, Baekjegobun-Ro 36-Gil, Songpa-Gu, Seoul, 05614, Korea • TEL : 82-2-420-0070 • FAX : 82-2-420-9797 • E-mail : unitma@unitma.com • Homepage : www.unitma.com w w w . u n i t m a . c o m
TissueMicroarray (TMA) Tissue Microarrays are a collection of multiple tissue cores that are arranged in columns and rows inside a paraffin block allowing for histological analysis. They are a crucial tool in the analysis of gene and protein expression levels in samples from normal and diseased specimens. Further, they are useful in the early-stage discovery of gene targets in genomic research, validating targets, testing, and optimization of diagnostic tests, and in the quality control of molecular detection schemes. And the TMA technology not only makes decrease the reagent, time, and human resource below one sixtieth but also can be applied to most of the know-how about tissues for immunohistochemistry, in situ hybridization, FISH and in situ PCR. TMAadvantages • The use of relatively small tissue cores to be positioned onto a unique slide allows simultaneous histological analysis of hundreds of samples. • High throughput screening of expression • Reduction of the number of slides to bemounted and analyzed → Time saving • Reduction of the amount of reagents and antibodies used → Money saving • Analysis of various biological specimens under uniform reaction conditions → Increasing the consistency of the results • Saving of precious biological samples and possibility to re-use donor blocks UNITMATMA technigue In conventional Tissue Microarray techniques, all the users have to prepare for their recipient blocks by drilling the dummy paraffin blocksmanually prior to startingTMAwork. But Unitma developed the premade paraffin recipient blocks for the first time in the world. The premade recipient blocks have thewell rounded holematrix suitable for the digital histologic analysis. Furthermore, Unitma’s premade recipient block brings to save the time and cost in preparation of the desired recipient blocksmanually and high throughput with a higher TMA success rate up to 99%. Based on the core technology, Unitma developed the manual and automated tissue microarrayers and has been supplying the most innovative instruments with the premade recipient blocks to worldwide customers as the best solution for the digital histology.Recently Unitma also launched the automated paraffin block trimmer which can trim the paraffin residues around the paraffin embedded tissue blocks without any damages to the sample tissues and any injuries during the trimmingwork by the conventional trimmingway. This instrument is only a paraffin block trimmer without any competitive instruments in the current worldmarket.Today, Unitma is continuously developing new technologies to change the existing histologic techniques. Premade Recipient Blocks AutomatedTissueMicroarrayer UATM-272B Manual TissueMicroarrayer Recipient BlockMold Kit AutomatedTissueMicroarrayer UATM-272A Paraffin BlockTrimmer 02 03 Certification Premade Recipient Block & Recipient Block Builder Manual TissueMicroarrayer AutomatedTissueMicroarrayer Paraffin BlockTrimmer ISO ISO ISO ISO CE CE CE CE FCC FCC UL unitma.com
04 05 Premade Recipient Blocks Unitma provides the premade recipient blocks patented in global to save the valuable time and cost in creating the recipient blocks additionally before startingTMAwork. The premade recipient block is made of special material which melts when heated at about 70 °C for 30~60minutes. The blocks have evenly spaced round wells arranged in a square matrix which conforms to digital pathology trend. Currently, six different sized blocks are available 0.5mm (320 holes), 1mm (120 holes), 1.5mm (90 holes), 2mm (60 holes), 3mm (30 holes), 5mm (20 holes) The blocks of 1, 1.5, 2, 3, 5mm applicable to manual Tissue Microarrayer while the blocks of 0.5, 1, 1.5, 2mm applicable to Automated Tissue Microarrayer (UATM-272B) Features • TMA success rate up to 99% • Maintains tissue integrity during embedding • Up to 300 tissue slices per a block • High durability duringmicroarraying • Standardized block configuration for the simple image analysis • Saving the additional time and cost in preparation of recipient blocks •Well rounded holematrix suitable for digital pathology • Ready-to-use paraffin recipient block • Compact design and versatility • No dedicated space required for TMA block formation In Situ Hybridization 0.5mm(320 holes) 1mm(120 holes) 1.5mm(90 holes) 2mm(60 holes) 3mm(30 holes) 5mm(20 holes) FISH Special stain Immunohistochemistry Product name Premade Recipient Block Model Number Core size Weight Number of cores UB06-0.5 0.5mm 3.4g 320 (16 x 20) holes UB06-1 1mm 3.2g 120 (10 x 12) holes UB06-1.5 1.5mm 2.8g 90 (9 x 10) holes UB06-2 2mm 2.5g 60 (6 x 10) holes UB06-3 3mm 2.5g 30 (5 x 6) holes UB06-5 5mm 1.8g 20 (4 x 5) holes Dimension 24 x 30 x 5.5mm Color and odor White, odorless Physical state and appearance Solid Storage temperature Room temperature Operating temparature 5°C ~35°C Composition Paraffin plus special materials Purpose of use Research purpose only Certification ISO, CE Specification Application unitma.com
06 07 Manual TissueMicroarrayer The newest technique for superior quality tissuemicroarrays (TMAs) The Quick Ray system represents the newest technique available for producing superior qualityTissueMicroarrays (TMAs) in less time than with traditional methods at a fractionof thecost. The Quick Ray technique produces superior quality TMAs in less time than with traditional methods, and accomplishes finished products at a fraction of the cost. The Quick Ray uses a hollowed tip to remove tissue cores as small as 1mm from paraffin-embedded tissue (donor block). The extracted tissue is then inserted into a preformed recipient block-the Quick Ray System is the only system available that uses a preformed paraffin recipientblock. The finished block is sectioned using a microtome; the sections are mounted on glass slides and then stained. Each TMA block can be cut into 300~400 sections at 4~5μ, which can then be subjected to independent tests. Common tests include immunohistochemistry and fluorescent in situ hybridization. Compact design and versatility make building a Quick Ray TMA astoundingly simple. The construction of a TMA block can be done on anycleancounter, anddoesnot requireadedicatedspace. Features • Portable and easy to handle • Shortening theTMAwork • Smarter arrayer compared to conventional products • Easy to carry and to make the array block anytime & anywhere • Inexperienced pathologist can be easily familiar with the kit • Simple procedure for creating the blocks • Easy to create the various sized blocks by using the premade recipient blocks • Save the time in preparation of the recipient blocks Recipient Blocks ChangeableTips 01 Place the reference slide and the donor block on the microscope stage, and mark the position with an oil pen, where you intend to extract the sample tissue. 04 Put the completed recipient block into the base mold with the face to be sectioned down and place it in an oven at 70 degrees celsius for 30 to 60 minutess until the block becomes completely transparent, then take it out from the oven. 02 Extract the marked tissue from the Donor block by Quick Ray 05 Place an embedding cassette on the top of transparent block and dispense liquid paraffin into the base mold until adequately covering the cassette. 06 Solidify the block in a cold plate 03 Deliver the extracted tissue into the corresponding holes of the recipient block supplied by UNIMTA 07 Sectioning by amicrotome 08 Follow the next steps How tobuild Specification Product name Manual TissueMicroarrayer (Quick Ray) Model name UT06 Components for a full set • 1 puncher body • 5 premade recipient blocks (1, 1.5, 2, 3, 5mm) • 5 puncher tips (1, 1.5, 2, 3, 5mm) • 1 tip guide for the 1mm recipient block • 1 basemold •Wooden case • User manual Dimension 220 x 163 x 45 (mm) Weight of a full set 0.83 kg Storage temperature Room temperature Operating temperature 5°C ~35°C Purpose of use Research purpose only Certifications ISO, CE unitma.com Quick Ray
08 09 AutomatedTissueMicroarrayer Quick Ray Master is an automated tissue microarrayer runningby thebuilt-inPC. The instrument extracts thesample tissues fromthedonor blocks and delivers the extracted sample tissues into the correspondent hole of the premade recipient block automatically. And the researcher can access to the data file for the current TMA work by using the Tissue Array Report Program(TARP) developedbyUNITMA. The researchers can choose one of the 3 rotary type tips simply by using uniqueUNITMA software. The instrument can be connected to the external devices such as USB, monitors, printers andetc. The instrument provides economical efficiency in time, quality, and cost of tissue preparation by fully automating tissuemicroarrayingprocess. Features • Analysisofmanypatient tissuesamples simultaneously • Conserves tumors, biopses and other precious biological samples • Conserves antibodies andother expensive reagents • Improves assay precision through sample and patient multi-plexing • Applicable tomRNAandproteinexpressionanalysis • Miniaturize and automate immunohistochemistry, in situhybridization, FISH, and insituPCR •High-speedpreparationof tissuemicroarrayblocks •Maintains tissue integrityduringembedding •Upto300 tissues slicesper recipientblock • Standardizedblockconfigurationsimply imageanalysis • Prepareupto240 tissuecoresper hour •TMAsuccess rateupto99% •Tissuearraycircle time15secs • Positional accuracyof 5μmat recipientblocks Key Facts Specification List Description Product name Quick RayMaster (Model : UATM-272A) Instrument type AutomatedTissueMicroarrayer Punch type Rotary puncher(1mm, 2mm, 3mm) Power supply 100 to 120VAC, 200 to 240VAC / 50~60Hz Space required 953 x 703 x 610mm (Wx D x H) Weight 148kg Stage capacity 10 donor blocks and 2 recipient blocks Speed 1 cycle time : 15 seconds (Pick & Place) Monitor LCDmonitor 15"(1024x768) with a touch screen Camera resolution Camera1 : 1280x1024, Camera2 : 2,592x1,944 (pixels) Operating system Unique UNITMA S/WbyMSWindowXP Recipient block Premade recipient block to be supplied by UNITMA Certifications ISO, CE, FCC, UL Quick RayMaster (Model: UATM-272A) Easy to use - Software based onMSWindowXP - Fully automated operation - Operation by touch screen - Automatically changeable punchers - Compact sizewith built-in PC, Monitor, Keyboard andMouse Productivity - 10 donor blocks and 2 recipient blocks at one time - Up to 240 cores of TMAwork in an hour Accuracy - Exact and detailedmovement control over 5 axes(X,Y,Z,P,R) Expandability - Access toTMAwork data - Networkingwith extertnal devices - Compatiblewith other application S/W Completion - Automatic data processing for traceability by Tissue Array Report Program(TARP) - Real timemonitoring of TMAwork on themonitor Flexibility - Operation by Auto or manual mode unitma.com
10 11 AutomatedTissueMicroarrayer Key Facts TARPfile Specification Product name Quick RayMaster (Model : UATM-272B) Instrument type AutomatedTissueMicroarrayer Tip type ChangeableTips (0.5mm, 1mm, 1.5mm, 2mm) Power supply 220VAC, 50/60Hz Dimension(WxDxH) 750 x 600 x 600 (mm) Weight 80kg Capacity 10 donor blocks and 2 recipient blocks Speed 1 cycle time : 10 seconds Camera resolution 2,592 x 1944 (pixels) Operating system Unique UNITMA S/WbyWindow7 Recipient block Unitma’s premade recipient blocks Certifications ISO, CE, FCC, UL Quick RayMaster (Model: UATM-272B) 1.Averyshorttimeforpreparationtooperatetheinstrument A user is not required to prepare the recipient blocks before starting the instrument since Unitma provide the premade recipientblocks. 2. Premade recipient blocks The 4 types of premade recipient block are applicable • 0.5mm-320holes (16x20) –World’s smallest hole size • 1mm-120 holes ( 10x12) • 1.5mm-90 holes( 9x10) • 2mm-60 holes( 6x10) 3. Powerful tipmodules The vacuum suctioning tips can extract the sample tissue from the marked area on the donor blocks by detecting with a high resolution camera, and insert the extracted sample tissue into the correspondent hole of the premade recipient block by detecting the center of the hole with the other high resolution camera. 4. The embedded unique S/W The Unitma’s S/Wcontrols themost functions of Quick RayMaster fully automatically. 5. Auto calibration and accurate movement of 4 axis to control thepunchersautomatically. 6. Auto orManual operation by user’s option 7. Networking with the external devices and compatible withotherapplicationS/W 8. Real time monitoring the current TMA work on the monitor 9. High productivity up to 240 holes per hour 10. Not needed the measurement of the donor block height 11. SmarterandeasierUser Interfacetoruntheinstrument. 12. EasyTMAdatamanagement by Excel data sheet Quick Ray Master is an automated tissue microarrayer running by simply connecting to user’s computer through USB port. The system extracts the sample tissues from the donor blocks and delivers the extracted sample tissues into the correspondent hole of the premade recipient block automatically. And the researcher can access the data file for the current TMA work by using theTissue Array Report Program (TARP) developed by UNITMA. The 4 different sized tips are manually changeable to associate with the desired core size of the recipient blocks: 0.5mm(320 holes), 1mm(120 holes), 1.5mm(90 holes), 2mm(60 holes). UATM-272B is smarter instrument compared to UATM-272A, and is designed for the users to be easily familiar with. The instrument provides economical efficiency in time, quality, and cost of tissue preparation by fully automating tissuemicroarraying process. Features • Analysisofmanypatient tissuesamples simultaneously • Conserves tumors, biopses and other precious biological samples • Conserves antibodies andother expensive reagents • Improves array precision through sample and patient multi-plexing • Applicable tomRNAandproteinexpressionanalysis • Miniaturize and automate immunohistochemistry, in situhybridization, FISH, and insituPCR •High-speedpreparationof tissuemicroarrayblocks •Maintains tissue integrityduringembedding •Upto300 tissues slicesper recipientblock • Standardizedblockconfigurationsimply imageanalysis • Prepareupto240 tissuecoresper hour •TMAsuccess rateupto99% •Tissuepreparationcycle timeof 15secs • Positional accuracyof 5μmat recipientblocks • Automaticcalibration unitma.com
12 13 Recipient BlockMoldKit Core size : 1mm / 170 holes (10 x 17) Core size : 3mm / 40 holes (5 x 8) Core size : 1.5mm / 150 holes (10 x 15) Core size : 5mm / 15 holes (3 x 5) Core size : 2mm / 70 holes (7 x 10) Recipient block mold kit is an alternative solution for the users to prepare the recipient block by themselves. The recipient block can be used to excuteTMAworks with themanual tissuemicroarrayer called Quick Ray. Furthermore, the researchers can prepare the hundreds of recipient blocks from a mold kit. For those who concerns over the price of premade recipient blocks, Quick-Ray Mold Kit is an alternative solution. Made from silicon rubber, Quick-Ray Mold kit allows users to build paraffin recipient blocks by themselves at a lower cost. The core sizes of each mold is perfectly match with punchers of Quick-Ray. If stored and handed with care, hundreds of paraffin recipient blocks can be produced by the Quick-Ray Mold Kit. But this recipient blocks can be applicable only for the manual tissue microarrayer. 01 Place the Recipient block Mold kit in a dry oven for 30 minutes at 70~80°C to warm-up themold kit. 04 Dispense enough liquid paraffin into the embedding cassette 02 Dispense liquidparaffin (60~65°C) slowly into the mold kit until the top of core rods are fully submerged. 05 Solidify the embedding cassette and the mold kit at a normal room temperature or at about 4°C for 30~60 minutes. If solidified at he lower temperature, the block may have cracks in it. A Paraffin dispense should be done so slowly that no bubbles are to be formulated among the core rods. B When some bubbles are formulated, removethem withheatedforceps. 06 Separate themold kit from the embedding cassette slowly and carefully. 03 Place an embedding cassette on themold kit. 07 Trimparaffin around the periphery of the recipient block. How tobuild unitma.com
14 15 ParaffinBlockTrimmer QuickTrim[UPBT-1011] Paraffin block before trimming Paraffin block after trimming Specification List Description Power supply voltages 110VAC / 220VAC, 50/60 Hz Weight (net) 47kg Max Size (Wx D x H) 580 x 477 x 280mm Operating temperature range Room temperature Relative humidity Max. 80%non-condensing Humidity during transportation/storage < 80% Trimming capacity Max. 750 blocks per hour Operating environment Indoor use only Certification / Approval ISO / CE / FCC The Paraffin Block Trimmer (generally called Wax Trimmer, Paraffin Trimmer, Wax Remover, Paraffin Remover, Dewaxer, etc) is one of the histology equipments used for the histopathology. The Paraffin Block Trimmer enables the researchers to trim the paraffin blocks automatically. This instrument removes only paraffin residues around the paraffin blocks without damage to the tissue samples or the plastic cassette. When users load the paraffin blocks on the inlet of the instrument after the embedding process, the blocks move into the trimming module to trim the paraffin residues and the trimmed blocks are arrayed by 50 blocks in order in the array plate. Eventually, this instrument also enables to reduce the manually trimming time and to prevent the users from any injuries caused by the conventional trimming tools. Features • Save labor time of researchers • Protect researchers from any physical damages which may be caused by conventional trimming tools. • Max. 750 paraffin blocks can be trimmed in an hour. • Semipermanant life time of trimmingmodules • Accept the various kinds of the embedded blocks. • 50 blocks can be trimmed at one time and continue trimming by replacing the array plate with a new one. • The trimmed blocks are arrayed in order at the array plate. unitma.com
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